The aim of this paper is to study the relationship betweenschool environment andachievement in biology at the higher secondary level.For present study,the researcher investigated the relationship between school environment and achievement inbiology of the students in Madurai District. The sample of the study consists of 200 higher secondary level biology students, selected by simple random technique. The investigator has used normative survey method for the study.The research tools used were ‘School Environment Inventory’ by Sabina Jose. S. and A. Edward William Benjamin. A. (2020) and Achievement test in Biology were adopted for the collection of data. The statistical techniques ‘t’ test, ANOVA and Pearson Correlationare used in testing the data.Findings of the study showed that there is no significant difference in gender, type of school and type of managementin relation to school environment and achievement in Biology

School Environment, Achievement in Biology, Higher Secondary Level