Introduction: Fine‐needle aspiration biopsy (FNAB) is a technique where a thin or a fine needle is inserted into a mass cellular material which is later aspirated. Cytological diagnosis is done on the aspirated material. FNAC is a simple, quick, inexpensive, and minimally invasive technique to diagnose different types of head and neck swellings. Aim: The main aim of this research is to understand the knowledge and awareness of the participants on aspiration biopsy. Materials and methods: A survey was designed to analyse the result. The questionnaire was prepared in Google Forms (online survey link) and was distributed to 100 dental undergraduate students. The questionnaire was based on fine needle aspiration biopsy. The responses were then collected and statistically analysed using SPSS software version 21.0. Descriptive analysis as well as Chi Square test was used to analyse the data. Results and discussion: Most participants were aware about aspiration biopsy (86%). There was a significant association between the year of study and the awareness on aspiration biopsy (P value=0.000). All groups of participants had knowledge about the contents of aspiration fluid and it showed no statistical significance (P Value=0.057) Conclusion: In the present study, the participants had adequate knowledge about aspiration biopsy which can be enhanced even more by various public surveys and spreading awareness.

FNAB or FNAC; aspiration, awareness, fine needle, innovative technique.