Aim The objective of the study is to compare the accuracy of bite marks on an inanimate substance (fruit) and a living tissue (skin) using digital analysis. Methodology A cross‑sectional study was conducted involving 15 volunteers. The registered bites of individuals on inanimate object (fruit) and living tissue (skin of forearm) were photographed with the American Board of Forensic Odontology scale No. 2 in the view field immediately after the production of bite marks. Dental casts of the individuals were obtained and photographed out of which computer‑assisted overlays were generated, and analysis was carried out digitally using Adobe Photoshop version 7. Statistical analysis was performed using IBM SPSS software, version 22. Results Skin had a comparable accuracy to that of an inanimate object which is statistically attested. Conclusion The source of bite marks, the substrate onto which they are generated and the technique of lifting the bite imprints serve as important tools in analysis.

American Board of Forensic Odontology guidelines, bite marks, Forensic odontology