Allium chinense (Chinese garlic) contains a variety of phytochemicals that are beneficial to health. Several extraction methods have been developed in order to achieve a high extraction yield. A pulse electric field was used in this study to aid extraction and reduce the use of harsh chemical solvents. The objective of this study was to investigate the effect of pulsed electric field (PEF)-assisted extraction of Chinese garlic on total phenolic content (TPC), total flavonoid content (TF), and biological activities like antioxidant activity (FRAP and DPPH), and anti-angiogenic activity. The study conditions varied PEF intensity (4, 5, and 6 kv/cm) with 1000, 3000, and 5000 pulses. As a control, Chinese garlics macerated in water were used. The results demonstrated that pulse electric fields have different effects on TPC and TFC. TPC value increased as the number of pulses increased, but electric field strength had no effect on TPC value. The garlic extracted at 5 kV/cm and 5000 pulses had the highest TPC value, measuring 56.46±3.64 mgGAE/100gFW. The FRAP assay revealed a positive correlation between antioxidant activity and TPC value, with a R value of 0.8647. TFC showed a distinct trend in which the values were affected by electric field strength but differed slightly when different pulse numbers were used. The TFC value was 18.72±0.43 mgQE/gFW when the garlic was treated with 6 kV/cm and 5000 pulses. Furthermore, the chick chorioallantoic membrane (CAM) assay was used to investigate the antiangiogenic activity of garlic extracts. The pattern of percent angiogenic inhibition followed the trend of total flavonoid content. It can be concluded that pulse intensity and pulse number had a positive effect on total phenolic and total flavonoid content extracted from garlic; however, different types of phytochemicals may be responsible for different biological activities.

Chinese garlic, phenolic content, flavonoid content, pulse electric field, biological activities