Aim:The investigation ofpresent study is to evaluate the Anticancer activity of leaf extract of Andrographispaniculata on human breast cancer MCF-7 cells. Materials and methods: The cytotoxic effect ofA.panniculatewas evaluated by 3-(4, 5-dimethyl thiazol-2 yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Further, the morphological assessment in MCF-7 cells upon treatment with/withoutA.paniculataleaf derived crude extract were examine under phase contrast microscope. All data obtained from the experiments were analysed and interpreted statistically (SPSS Student Version Software Package; SPSS Inc., Chicago, IL, USA) using student t-test and one-way ANOVA

Cytotoxicity, MCF-7, Andrographispaniculata, MTT assay