Research Article | Open Access
ANTI CANCER SCREENING OF CARDIOSPERMUM HALICACABUM LEAF EXTRACT ON HUMAN BREAST CANCER CELL LINE
Joseph George Kallivayalil,Dr. S. Raghunandhakumar,Dr.D. Ezhilarasan,Dr. T. Lakshmi
Pages: 8056-8061
Abstract
BACKGROUND: To investigate the in vitro antioxidant and anticancer activity of chloroform and ethanol extracts of Cardiospermumhalicacabum leaves.
AIM: The objective of the study was to examine the cytotoxic activity of leaf extract of Cardiospermumhalicacabumon MCF-7 human breast cancer cell line.
METHODS:The cytotoxic activity of Cardiospermum was measured by MTT. Briefly, the cells (1 × 105 cells per ml)were seeded in a 96 well microtiter plate (100 μl per well) with three individual experiments. Treatment was conducted with different concentrations (20, 40, 60,80,100,200mg/ml) of cardiospermum extract for 24hrs.Further the apoptotic effect was evaluated with morphological examination using phase contrast microscopy
Keywords
Cardiospermum ;MCF-7; cancer; innovative study