Research Article | Open Access
A comparative study of Ayoub-Shklar, Dane-Herman, Alcian blue-PAS, Rapid Papanicolaou stain, Grams stain with hematoxylin-eosin stain for demonstration of keratin in paraffin-embedded tissue sections
Amisha Kakkar, Karthikeyan Ramalingam, Manish Agarwal , Vineet Tyagi , Mamta Tanwar , Suddhasattva Bose
Pages: 10419-10428
Abstract
Aim:
The aim of the present study was to compare the efficacy of special stains for keratin with standard hematoxylin and eosin stain (H and E).
Objectives:
(i) To use special stains - Ayoub-Shklar method, Dane-Herman method, Alcian blue –
periodic acid Schiff’s (PAS), rapid Papanicolaou (PAP) and Gram’s stain among
different archival cases.
(ii) To compare the staining specificity and staining intensity of special stains with
respect to routine hematoxylin and eosin (H and E) stain
Keywords
Special stains, Ayoub-Shklar, Dane-Herman, Alcian blue-periodic acid Schiff, PAP, Grams